tree.dndController.singular = true;
これが正しいかは、ちょっと怪しいが、自分でも試したら動いた。
dojo - How to disable multiple selection of nodes in dijit.Tree? - Stack Overflow
var tree = new dijit.Tree({ id: "tree1", model: model }).placeAt(win.body()); tree.dndController.singular = true;